Possible Sources of Error in Enzyme Catalase Lab

AP Bio Enzyme Lab. Class practical or demonstration.

Catalase Lab A Bio Enzyme Activity Investigation

Hydrogen peroxide bubbles when it comes into contact with an enzyme called catalase.

. Repeat steps 1-3 using the new measurements as described in table 1. The catalase sample for todays lab will be from a potato. In this lab we will use potatoes as our catalase source.

In this part of the lab we were able to easily see the effect of sulfuric acid on the enzyme catalase even if all our other data was messed up. Physical and chemical laboratory experiments include three primary sources of error. Change in Enzyme Concentration.

2 H2O2 liquid -- Catalase 2 H2O. Seal test tube A1 with a stopper immediately after the enzyme is added. Enzymes are proteins that catalyze chemical reactions increase the rates of biological reactionsThe reaction that will be investigated in this experiment is the decomposition of hydrogen peroxide.

Annie ChiangIB Biology HLPeriod 2Catalase Activity LabIntroduction. The x and y intercept makes sense at 00 as when there is no hydrogen peroxide in a solution of the catalase the catalase is obviously not going to be making the reaction move forward in any rate. Sources of Error Throughout this lab there were many different possible sources of error.

Enzymes speed the rate of chemical reactions. Catalase lab report 1. Pour 5ml of substrate 0ml of enzyme no enzyme for negative control and 5ml of water into test tube A1.

Additional Information that May Help. The enzyme that helps with this reaction is called catalase. Not only was the procedure itself not completely accurate but the equipment was also faulty.

In this practical liver was used as a source of the enzyme catalase. The organelle responsible for destroying hydrogen peroxide is the peroxisome using the enzyme catalase. Catalase can be purchased as a dessicated powder from biological supply companies See Appendix A.

The reaction equation is. Store the substance as received in a freezer until used. Systematic error random error and human error.

Up to 24 cash back This was most likely due to a source of error namely slowly screwing on the lid within the lab. The mL of oxygen produced with increase of catalase 30secs 60secs. There were a few possible sources of error in the lab.

The limiting factor for the reaction is the speed of the enzyme. Table 1 Substrate Enzyme. The catalase enzyme stock solution is prepared on the day of the laboratory using the following general formula.

80 concentration 8 mL potato juice 2 mL distilled water 8 cm. These rates remained the average for Part B. Because we were working with such a small volume of liquid the concentration of the the contents could have easily swayed the results.

Hydrogen peroxide H 2 O 2 is a by-product of respiration and is made in all living cells. Purpose The purpose of this catalase lab is to design simple experiments to demonstrate how various factors affect the rate of enzyme activity. It was hypothesised that if the concentration of the substrate hydrogen peroxide is increased then the rate of enzyme activity will increase to a point of saturation then remain at a constant rate.

Bias Busters Prezi Video. Many of the testing bottles that were mismatched with their lids. This lab shows how the enzyme decomposes in hydrogen peroxide.

This is found in both plants and animals. A catalyst is a chemical involved in but not consumed in a chemical. 1 Precision of your instruments none are perfect 2 Random errors contamination 3 You cant control all variables genetic.

The average reaction rates for Part A were 002 to 003 mLsec oxygen gas added to the test tube. Hydrogen peroxide H 2 O 2 degrades over time and the concentration enzyme solution may not have been exact. Cells make the enzyme catalase to remove hydrogen peroxide.

Because of this the enzyme denatured since a change in pH level either too high or too low can have denaturing effects on an enzyme. Up to 24 cash back Sources of Error. Hydrogen peroxide is harmful and must be removed as soon as it is produced in the cell.

2H2O2 2H2O O2. Both plants and animals have peroxisomes with catalase. This investigation looks at the rate of oxygen production by the catalase in pureed potato as the.

These sources of errors in lab should be studied well before any further action. Sulfuric acid lowers the pH level of the total solution. Record the reaction rate using the Logger Pro software.

Highlights in hybrid learning. One source of error could have occurred when the potato slices were placed into the the brown bottle being that oxygen could have been lost. What would be unavoidable sources of error in a lab experiment.

Methods and Materials Refer to handout attached to the back of lab Observations Table 1. Concentration needed X volume needed units mg X mg.

Part 3 Enzyme Concentration And The Rate Of Catalase Activity

Catalase Lab

Doc Investigating Factors That Affect The Rate Of Enzyme Activity Sandra Tom Academia Edu